CAMAG® TLC Scanner 4

• Measurement of reflection, either in absorbance and/or fluorescence
• Spectral range from 190 to 900 nm
• Data step resolution 25–200 μm
• Spectrum recording up to 100 nm/s
• Any plate format up to 20 x 20 cm
• Software-controlled by visionCATS

The Optical System

• Any of the three light sources, high pressure mercury lamp, deuterium lamp, or halogen-tungsten lamp can be positioned in the light path by a motor drive. (1)
• The signal of the measuring photomultiplier is continuously offset against the signal of the reference photomultiplier. (4 and 5) This compensates for lamp aging and short-time fluctuations. It also reduces the warm-up time required to reach lamp stabilization.
• All components of the optical system, lamps, monochromator, scanning stage, and photomultiplier are mounted on one sturdy metal support. This ensures high precision of the detector signal.
• For scanning at wavelengths below 200 nm it is advisable to flush the monochromator with nitrogen. The TLC Scanner 4 is equipped to do this.
• A monochromator bandwidth of 5 nm or 20 nm can be selected. 5 nm bandwidth is used for spectra recording, multi-wavelength scanning, and when spectral selectivity is required. (2) 20 nm bandwidth offers higher light intensity (improves the signal to noise ratio and thus the reproducibility of the measurement) and enables measurement of several fractions with slightly different absorption maxima in one scan.
• The lens system with 190 – 900 nm transmission range features automatic positioning for micro and macro slit sizes. This ensures that the light energy available with small slits in the micro position is almost the same as that for the corresponding slit in the macro position, which is four times larger. (3)
• The light beam strikes the object at right angle. The photomultiplier is aligned at an angle of 30°. (5)

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